Biological Pretreatment of Softwood Pinus densiflora by Three White Rot Fungi
이재원, 곽기섭, 박준영, 박미진, 최돈하, 권미, 최인규,
소속 상세정보
이재원 ( Lee Jae-Won )
서울대학교 농업생명과학대학 산림과학부
곽기섭 ( Gwak Ki-Seob )
서울대학교 농업생명과학대학 산림과학부
박준영 ( Park Jun-Yeong )
서울대학교 농업생명과학대학
박미진 ( Park Mi-Jin )
서울대학교 농업생명과학대학 산림과학부
최돈하 ( Choi Don-Ha )
국민대학교 삼림과학대학
권미 ( Kwon Mi )
국민대학교 삼림과학대학
최인규 ( Choi In-Gyu )
서울대학교 농업생명과학대학
KMID : 0880220070450060485
Abstract
The effects of biological pretreatment on the Japanese red pine Pinus densiflora, was evaluated after exposure to three white rot fungi Ceriporia lacerata, Stereum hirsutum, and Polyporus brumalis. Change in chemical composition, structural modification, and their susceptibility to enzymatic saccharification in the degraded wood were analyzed. Of the three white rot fungi tested, S. hirsutum selectively degraded the lignin of this sortwood rather than the holocellulose component. After eight weeks of pretreatment with S. hirsutum, total weight loss was 10.7%, while lignin loss was the highest at 14.52% among the tested samples. However, holocellulose loss was lower at 7.81% compared to those of C. lacerata and P. brumalis. Extracelluar enzymes from S. hirsutum showed higher activity of ligninase and lower activity of cellulase than those from other white rot fungi. Thus, total weight loss and changes in chemical composition of the Japanese red pine was well correlated with the enzyme activities related with lignin- and cellulose degradation in these fungi. Based on the data obtained from analysis of physical characterization of degraded wood by X-ray Diffractometry (XRD) and pore size distribution, S. hirsutum was considered as an effective potential fungus for biological pretreatment. In particular, the increase of available pore size of over 120 nm in pretreated wood powder with S. hirsutum made enzymes accessible for further enzymatic saccharification. When Japanese red pine chips treated with S. hirsutum were enzymatically saccharified using commercial enzymes (Cellulclast 1.5 L and Novozyme 188), sugar yield was greatly increased (21.01%) compared to non-pretreated control samples, indicating that white rot fungus S. hirsutum provides an effective process in increasing sugar yield from woody biomass.
키워드
biological pretreatment;white rot fungi;enzymatic saccharification;woody biomass;ligninase;available pore size
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