Proteomic Analysis of Outer Membrane Proteins from Acinetobacter baumannii DU202 in Tetracycline Stress Condition
윤성호, 이제철, 임선희, 김승일, 최종순, 김수현, 최치원, 박순호,
소속 상세정보
윤성호 ( Yun Sung-Ho )
한국기초과학지원연구원 Proteomics Team
이제철 ( Lee Je-Chul )
경북대학교 의과대학 미생물학교실
임선희 ( Leem Sun-Hee )
동아대학교 자연과학대학 생명과학부
김승일 ( Kim Seung-Il )
한국기초과학지원연구원 Proteomics Team
최종순 ( Choi Jong-Soon )
기초과학지원연구소
김수현 ( Kim Soo-Hyun )
한국기초과학지원연구원 Proteomics Team
최치원 ( Choi Chi-Won )
한국기초과학지원연구원
박순호 ( Park Soon-Ho )
한국기초과학지원연구원 Proteomics Team
KMID : 0880220080460060720
Abstract
Acinetobacter baumannii readily developed antimicrobial resistance to clinically available antibiotics. A. baumannii DU202 is a multi-drug resistant strain, and is highly resistant to tetracycline (MIC>1,024μg/ml). The surface proteome of A. baumannii DU202 in response to the sub-minimal inhibitory concentration (subMIC) of tetracycline was analyzed by 2-DE/MS-MS and 1-DE/LC/MS-MS to understand the pathways that form barriers for tetracycline. Membrane expression of major outer membrane proteins (Omps) was significantly decreased in response to the subMIC of tetracycline. These Omps with sizes of 38, 32, 28, and 21 kDa were identified as OmpA38, OmpA32, CarO, and OmpW, respectively. However, transcription level of these Omps was not significantly changed. 1-DE/LC/MS-MS analysis of secreted proteins showed that OmpA38, CarO, OmpW, and other Omps were increasingly secreted at tetracycline condition. This result suggests that A. baumannii actively regulates the membrane expression and the secretion of Omps to overcome antibiotic stress condition.
키워드
Acinetobacter baumannii DU202;outer membrane proteins (Omps);multi-drug resistant (MDR)
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