Mongolian Gerbil에서 유발시킨 일과성 전뇌허혈로 인한 Polyamine 함량변동과 해마신경세포손상에 대한 Melatonin 투여의 억제작용
Effect of Melatonin on Brain Polyamine Contents and Hippocampal Neuronal Damage after Transient Global Ischemia in Mongolian Gerbil
대한뇌혈관학회지 2002년 4권 1호 p.46 ~ 51
장영식, 서영성, 이성룡, 김대현, 임만빈,
소속 상세정보
장영식 ( Jang Young-Sik )
계명대학교 의과대학 가정의학교실
서영성 ( Suh Young-Sung )
계명대학교 의과대학 가정의학교실
이성룡 ( Lee Seung-Ryong )
계명대학교 의과대학 약리학교실
김대현 ( Kim Dae-Hyun )
계명대학교 의과대학 동산의료원 가정의학교실
임만빈 ( Yim Man-Bin )
계명대학교 의과대학 신경외과학교실
KMID : 0890520020040010046
Abstract
Objectives: This study was designed to examine whether melatonin has a neuroprotective effect against hippocampal neuronal damage following transient global ischemia in a gerbil. Polyamine is known to play a role in the pathophysiology of ischemic neuronal damage, we evaluated the influences of melatonin on the polyamine level as well as histology.
Materials and Methods: Male Mongolian gerbils (60-80 g) were used in this study. Transient global ischemia was induced by occlusion of the bilateral common carotid arteries for 3 min with microclips. Melatonin was administered immediately after occlusion. The animals were decapitated 24 h after the occlusion for polyamine measurement by a high performance liquid chromatography (HPLC) and 4 days after the occlusion for histological evaluation (hematoxylin and eosin staining). A histological examination was performed by a blinded investigator.
Results: The hippocampal putrescine level increased compared to sham-operated animals and the increase of putrescine was attenuated by 20 mg/kg melatonin administration. Spermidine and spermine levels didn’t show significant changes after ischemia. Hippocampal neuronal damage in the CA1 region was markedly observed in vehicle-treated animals compared to sham-operated animals. Melatonin administration (10 or 20 mg/kg) significantly inhibited hippocampal CA1 neuronal damage after ischemia compared to corresponding vehicle-treated animals (p<0.05 and p<0.01, respectively).
Conclusions: Melatonin attenuates the putrescine level after transient global ischemia and may have putative neuroprotective effects against global ischemia induced neuronal damage.
키워드
전뇌허혈;Gerbil;Polyamine;해마;신경세포 손상;Melatonin
Global ischemia;Gerbil;Polyamine;Hippocampus;Neuronal damage;Melatonin
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