신장 암 세포에서 Transient Receptor Potential Melastatin-Subfamily Member 7 억제에 의한 Tissue Inhibitors of Metalloproteinase 발현의 변화
Changes of Tissue Inhibitors of Metalloproteinase Expression by Transient Receptor Potential Melastatin-Subfamily Member 7 Suppression in Renal Cancer Cells
김덕윤, 김순희, 양은경,
소속 상세정보
김덕윤 ( Kim Duk-Yoon )
Catholic University of Daegu School of Medicine Department of Urology
김순희 ( Kim Soon-Hee )
Kyungpook National University School of Medicine Department of Physiology
양은경 ( Yang Eun-Kyoung )
Kyungpook National University School of Medicine Department of Physiology
Abstract
Purpose: Recent studies have shown that transient receptor potential melastatin-subfamily member 7 (TRPM7) may enhance cancer cell growth, migration and invasion of human renal cell carcinoma (RCC). We investigated how TRPM7 regulated progression of RCC by interacting with matrix metalloproteinase (MMP)/tissue inhibitors of metalloproteinase (TIMP) pathway.
Materials and Methods: We performed a wound healing assay and a transwell migration to examine the migration of RCC cells and transwell invasion assay to assess the invasion of RCC cells. Western blot analysis was used to show the expression of MMP-2, MMP-9, TIMP-1, and TIMP-2.
Results: The migration and invasion of RCC cells were markedly suppressed by siRNA targeting TRPM7. Lowering of TRPM7 increased MMP-2 expression and induced no change in MMP-9 expression. Strikingly, TRPM7 silencing suppressed the expression of TIMP-1 and TIMP-2.
Conclusions: These results suggested that MMP-independent action of TIMPs may take part in the enhancing effect of TRPM7 on the progression of RCC.
키워드
Transient receptor potential melastatin-subfamily member 7; Renal cell cancer; Tissue inhibitor of metalloproteinase
원문 및 링크아웃 정보
등재저널 정보