Preparation of radiolabeled polycyclic aromatic hydrocarbon assemblies for biological assessment of diesel exhaust particulates
이창헌, Shim Ha-Eun, Song Lee, 전종호,
소속 상세정보
이창헌 ( Lee Chang-Heon )
Korea Atomic Energy Research Institute Advanced Radiation Technology Institute
( Shim Ha-Eun )
Korea Atomic Energy Research Institute Advanced Radiation Technology Institute
( Song Lee )
Korea Atomic Energy Research Institute Advanced Radiation Technology Institute
전종호 ( Jeon Jong-Ho )
Korea Atomic Energy Research Institute Advanced Radiation Technology Institute
Abstract
The potential health risk from inhalational exposure of diesel exhaust particulates (DEP) has gained considerable scientific interests. However, the long-term in vivo behavior of DEP have not been clearly understood due to the difficulty of accurate analysis of these substances in a living subject. We herein demonstrate a detail protocol for the preparation of radiolabeled DEP using a radioactive-iodine-tagged pyrene analog. The purified 125I-labeled pyrene ([125I]1) was obtained with a good radiochemical yield (32 ± 4%, n=3) and high radiochemical purity (>99%) from the stannylated precursor 2. Next, the purified [125I]1 was successfully assembled into the DEP suspension in an efficient manner. The radiolabeled DEP was highly stable in a mouse serum for 7 days without significant deiodination or dissociation of [125I]1. These results clearly indicate that the present radiolabeling method will be useful for biodistribution study of carbonaceous particulates in vivo.
키워드
Radiolabeling; Radioactive iodine; PAHs; DEP
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